Table 1 Technical information and treatment sequence for the three regenerative endodontic cases reported in this article

1

Local anesthesia 4% articaine 1:100,000 epinephrine

Local anesthesia 2% lidocaine without vasoconstrictor

2

Rubber dam isolation and access opening

Rubber dam isolation and re-access opening

3

Irrigation with 10 mL 2.5% sodium hypochlorite (NaOCl)

Irrigation with 10 mL EDTA to remove the calcium hydroxide (Ultracal^®)

4

Working length (periapical radiography) and gentle mechanical preparation in the coronal and middle canal thirds

Irrigation with 10 mL saline solution

5

Re-irrigation with 10 mL 2.5% NaOCl with 27-G needle tip

Drying with absorbent paper points

6

Irrigation with 10 mL EDTA

Bleeding induction with K-file size #40, gentle movements up to 2 mm beyond the root apex

7

Irrigation with 10 mL of 2.5% NaOCl

Determination of bleeding time (s)

8

Irrigation with 10 mL of saline

Application of the total syringe (0.15 mL) of EMD (Emdogain^®) mixing with blood

9

Drying with absorbent paper points

Placement and condensation of white MTA in the coronal third of root canals

10

Insertion of calcium hydroxide (Ultracal^®)

Base of restorative glass ionomer cement

11

Insertion of a sterile cotton pellet

Definitive restoration with composite resin

12

Temporary restoration (Coltosol^® + Riva Light Cure restorative glass ionomer cement)

Radiograph to confirm MTA placement/adaptation